LOCUS       40924_27044       10171 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pKT pKT-NB-H2BsfGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Kakimoto T, Tsujimura K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Department of Biological Sciences, Graduate 
            School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, 
            Osaka 560-0043, Japan
REFERENCE   2  (bases 1 to 10171)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-SEP-2018) Department of Biological Sciences, Graduate School of 
            Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 
            560-0043, Japan"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(279..303)
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     CDS             complement(826..1614)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      complement(2219..2413)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2482..3552)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3984..4610)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    5945..6085
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6270..6858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7503..7527)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        7663..7842
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7893..8441
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      8475..8675
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     primer_bind     complement(8688..8704)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8724..8761
                     /label=multi cloning site
                     /note="multi cloning site"
     misc_feature    9197..9208
                     /label=4x glycine linker
                     /note="4x glycine linker"
     CDS             9209..9922
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     misc_feature    9926..9940
                     /label=multi cloning site
                     /note="multi cloning site"
     regulatory      9941..10134
                     /label=terminator of CaMV 35S
                     /note="terminator of CaMV 35S"
                     /regulatory_class="terminator"
ORIGIN
        1 gccgatcgtg aagtttctca tctaagcccc catttggacg tgaatgtaga cacgtcgaaa
       61 taaagatttc cgaattagaa taatttgttt attgctttcg cctataaata cgacggatcg
      121 taatttgtcg ttttatcaaa atgtactttc attttataat aacgctgcgg acatctacat
      181 ttttgaattg aaaaaaaatt ggtaattact ctttcttttt ctccatattg accatcatac
      241 tcattgctga tccatgtaga tttcccggac atgaagccat ttacaattga atatatcctg
      301 ccgccgctgc cgctttgcac ccggtggagc ttgcatgttg gtttctacgc agaactgagc
      361 cggttaggca gataatttcc attgagaact gagccatgtg caccttcccc ccaacacggt
      421 gagcgacggg gcaacggagt gatccacatg ggacttttaa acatcatccg tcggatggcg
      481 ttgcgagaga agcagtcgat ccgtgagatc agccgacgca ccgggcaggc gcgcaacacg
      541 atcgcaaagt atttgaacgc aggtacaatc gagccgacgt tcacgcggaa cgaccaagca
      601 agctatgttg cgattacttc gccaactatt gcgataacaa gaaaaagcca gcctttcatg
      661 atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact
      721 tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag
      781 ccgcgccgcg aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt
      841 ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa
      901 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
      961 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
     1021 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
     1081 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
     1141 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
     1201 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
     1261 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
     1321 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
     1381 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     1441 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
     1501 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     1561 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
     1621 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1681 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1741 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1801 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1861 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1921 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1981 ggtttcggtc tccacgcatc gtcaggcata cccaccggtg ccttgatgtg ggcgccggcg
     2041 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     2101 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     2161 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     2221 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     2281 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     2341 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     2401 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     2461 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     2521 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     2581 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     2641 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     2701 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     2761 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     2821 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     2881 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     2941 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     3001 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     3061 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     3121 cgccatcagt accaggtcgt aatcccacac actcgccatg ccggccggcc ctgcggaaac
     3181 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     3241 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     3301 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     3361 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     3421 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgccgcctt
     3481 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     3541 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     3601 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     3661 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     3721 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     3781 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     3841 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     3901 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3961 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     4021 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     4081 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     4141 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     4201 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     4261 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     4321 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     4381 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     4441 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     4501 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     4561 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     4621 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     4681 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     4741 tcgccggcca ggccgcgagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     4801 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     4861 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     4921 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4981 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     5041 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     5101 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     5161 aaacttcacg cctcgcgcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     5221 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     5281 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     5341 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     5401 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     5461 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     5521 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     5581 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     5641 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     5701 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     5761 tgcactatag cagcggaggg gttggatcga cctcgacgta cccctgcctc gcgcgtttcg
     5821 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5881 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5941 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     6001 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6061 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tcggttatcc
     6181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6961 atccttttcg gcgtccacat caacggcgtc ggcggcgact gcccaggcaa gaccgagatg
     7021 caccgcgata tcttgctgcg ttcggatatt ttcgtggagt tcccgccaca gacccggatt
     7081 gaaggcgaga tccagcaact cgcgccagat catcctgtga cggaactttg gcgcgtgatg
     7141 actggccagg acgtcggccg aaagagcgac aagcagatca cgcttttcga cagcgtcgga
     7201 tttgcgatcg aggatttttc ggcgctgcgc tacgtccgcg accgcgttga gggatcaagc
     7261 cacagcagcc cactcgacct tctagccgac ccagacgagc caagggatct ttttggaatg
     7321 ctgctccgtc gtcaggcttt ccgacgtttg ggtggttgaa cagaagtcat tatcgcacgg
     7381 aatgccaagc actcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
     7441 gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt
     7501 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaaccgaag gcgggaaacg
     7561 acaatctgat cgggtaccgg atcatgagcg gagaattaag ggagtcacgt tatgaccccc
     7621 gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg
     7681 agccactcag ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt
     7741 attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa
     7801 tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca tattcactct
     7861 caatccagat ctcgactcta gggggatcta ccatgagccc agaacgacgc ccggccgaca
     7921 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
     7981 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
     8041 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
     8101 gcatcgccta cgcgggcccc tggaaggcac gcaacgccta cgactggacg gccgagtcga
     8161 ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc tacacccacc
     8221 tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca
     8281 acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc atgctgcggg
     8341 cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg gacttcagcc
     8401 tgccggtacc gccccgtccg gtcctgcccg tcaccgagat ctgatgaccc ctagaggatc
     8461 ccccgatgag ctaagctagc tatatcatca atttatgtat tacacataat atcgcactca
     8521 gtctttcatc tacggcaatg taccagctga tataatcagt tattgaaata tttctgaatt
     8581 taaacttgca tcaataaatt tatgtttttg cttggactat aatacctgac ttgttatttt
     8641 atcaataaat atttaaacta tatttctttc aagatgggaa ttaattcact ggccgtcgtt
     8701 ttacacatgg cggccgcgtc gacatcgata agcttggcgc gccatcctgc aggccctcga
     8761 gatggcgaag gcagataaga aaccagcgga gaagaaaccg gcagagaaaa ctccggcagc
     8821 cgaaccagca gcagcggcag agaagaaacc aaaagccgga aagaaactcc cgaaggaacc
     8881 agccggcgcc ggagacaaga agaagaagag atcaaagaag aacgttgaga catacaagat
     8941 ctacatcttc aaggtgttga agcaagttca tccagatatc ggaatctcca gcaaagccat
     9001 gggaatcatg aacagtttca tcaatgatat ctttgagaaa cttgctggtg agtcttcgaa
     9061 gcttgcgagg tacaacaaga agccgacgat tacttctagg gagattcaga ctgcggtgag
     9121 acttgtgttg cctggagagt tggcgaaaca tgctgtttct gaagggacta aggcggttac
     9181 gaagtttacg agttctggag gtggaggtat gtctaaaggt gaagagcttt tcacaggagt
     9241 tgtgccaatc ttggttgagt tggatggtga cgttaatgga cataagttct ctgttagagg
     9301 agaaggagaa ggagatgcta ccaatggaaa gctcacgttg aagttcatct gcacaactgg
     9361 aaaattgcca gttccatggc ctactcttgt taccactctt acttacggag ttcagtgctt
     9421 ctctagatac ccagaccaca tgaaacgaca tgatttcttc aaatctgcta tgcctgaagg
     9481 atatgtgcaa gagaggacca tctctttcaa agacgatggc acttacaaga caagagctga
     9541 agtgaagttt gaaggagata cccttgtgaa caggatagaa cttaaaggca tcgactttaa
     9601 agaggatgga aacatattgg gacataaact cgaatataac ttcaatagcc acaatgtcta
     9661 cattacagcc gataagcaga agaatggaat caaagctaac tttaagattc gtcacaacgt
     9721 agaggatgga agtgtgcaac ttgctgacca ttaccaacag aatacaccta ttggtgatgg
     9781 acctgtcctt ttgccagata accactatct ctccacgcaa tcagttctct ctaaagatcc
     9841 aaatgagaaa cgggatcata tggtcttgct tgagtttgtt acagctgctg gaattactca
     9901 tggaatggac gaattgtaca aatgacccgg gatttaaatc accagtctct ctctacaaat
     9961 ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag
    10021 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
    10081 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacct
    10141 gcagactagt aaggccttaa gggcctctag a
//
